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    Intracellular uptake of Pluronic copolymer: effects of the aggregation state

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    Date
    2002
    Author
    Muniruzzaman, Md.
    Marin, Alexander
    Luo, Yi
    Prestwich, Glenn D.
    Pitt, William G.
    Husseini, Ghaleb
    Rapoport, Natalya Y.
    Advisor(s)
    Unknown advisor
    Type
    Peer-Reviewed
    Article
    Postprint
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    Abstract
    The effect of the Pluronic P-105 aggregation state on its uptake by HL-60 cells was studied by flow cytometry, fluorescence spectroscopy, and confocal and fluorescence microscopy using a fluorescently labeled Pluronic P105. In the low concentration region below the critical micelle concentration (CMC), Pluronic uptake was proportional to the concentration in the incubation medium. The proportionality broke sharply above the CMC, revealing a less efficient intracellular uptake of Pluronic micelles than that of unimers. The data suggested that Pluronic micelles were internalized via fluid-phase endocytosis while unimers were internalized via diffusion through plasma membranes. Based on the above findings, the shielding effect of Pluronic micelles on drug intracellular uptake was explained.
    DSpace URI
    http://hdl.handle.net/11073/19831
    External URI
    https://doi.org/10.1016/S0927-7765(01)00329-0
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