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dc.contributor.authorBuchanan, Clara M.
dc.contributor.authorWood, Ryan L.
dc.contributor.authorHoj, Taalin R.
dc.contributor.authorAlizadeh, Mahsa
dc.contributor.authorBledsoe, Colin G.
dc.contributor.authorWood, Madison E.
dc.contributor.authorMcClellan, Daniel S.
dc.contributor.authorBlanco, Rae
dc.contributor.authorHickey, Caroline L.
dc.contributor.authorRavsten, Tanner V.
dc.contributor.authorHusseini, Ghaleb
dc.contributor.authorRobison, Richard A.
dc.contributor.authorPitt, William G.
dc.date.accessioned2021-02-02T10:10:50Z
dc.date.available2021-02-02T10:10:50Z
dc.date.issued2017
dc.identifier.citationClara M. Buchanan, Ryan L. Wood, Taalin R. Hoj, Mahsa Alizadeh, Colin G. Bledsoe, Madison E. Wood, Daniel S. McClellan, Rae Blanco, Caroline L. Hickey, Tanner V. Ravsten, Ghaleb A. Husseini, Richard A. Robison, William G. Pitt, Rapid separation of very low concentrations of bacteria from blood, Journal of Microbiological Methods, Volume 139, 2017, Pages 48-53, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2017.05.004.en_US
dc.identifier.issn0167-7012
dc.identifier.urihttp://hdl.handle.net/11073/21317
dc.description.abstractA rapid and accurate diagnosis of the species and antibiotic resistance of bacteria in septic blood is vital to increase survival rates of patients with bloodstream infections, particularly those with carbapenem-resistant enterobacteriaceae (CRE) infections. The extremely low levels in blood (1 to 100 CFU/ml) make rapid diagnosis difficult. In this study, very low concentrations of bacteria (6 to 200 CFU/ml) were separated from 7 ml of whole blood using rapid sedimentation in a spinning hollow disk that separated plasma from red and white cells, leaving most of the bacteria suspended in the plasma. Following less than a minute of spinning, the disk was slowed, the plasma was recovered, and the bacteria were isolated by vacuum filtration. The filters were grown on nutrient plates to determine the number of bacteria recovered from the blood. Experiments were done without red blood cell (RBC) lysis and with RBC lysis in the recovered plasma. While there was scatter in the data from blood with low bacterial concentrations, the mean average recovery was 69%. The gender of the blood donor made no statistical difference in bacterial recovery. These results show that this rapid technique recovers a significant amount of bacteria from blood containing clinically relevant low levels of bacteria, producing the bacteria in minutes. These bacteria could subsequently be identified by molecular techniques to quickly identify the infectious organism and its resistance profile, thus greatly reducing the time needed to correctly diagnose and treat a blood infection.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.urihttps://doi.org/10.1016/j.mimet.2017.05.004en_US
dc.subjectBlood sepsisen_US
dc.subjectBacterial infectionen_US
dc.subjectRapid diagnosisen_US
dc.subjectE. colien_US
dc.subjectCarbapenem-resistant enterobacteriaceaeen_US
dc.subjectSedimentationen_US
dc.titleRapid separation of very low concentrations of bacteria from blooden_US
dc.typePeer-Revieweden_US
dc.typeArticleen_US
dc.typePostprinten_US
dc.identifier.doi10.1016/j.mimet.2017.05.004


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