Show simple item record

dc.contributor.authorMiller, Jessica R.
dc.contributor.authorSiripurkpong, Pilaiwan
dc.contributor.authorHawes, Jennifer
dc.contributor.authorMajdalawieh, Amin
dc.contributor.authorRo, Hyo-Sung
dc.contributor.authorMcLeod, Roger S.
dc.date.accessioned2022-11-28T07:57:11Z
dc.date.available2022-11-28T07:57:11Z
dc.date.issued2008
dc.identifier.citationMiller, J.; Siripurkpong, P.; Hawes, J.; Majdalawieh, A.; Ro, H.; McLeod, R. The trans-10, cis-12 isomer of conjugated linoleic acid decreases adiponectin assembly by PPARγ-dependent and PPARγ-independent mechanisms, Journal of Lipid Research, Volume 49, Issue 3, 2008, Pages 550-562, ISSN 0022-2275, https://doi.org/10.1194/jlr.M700275-JLR200.en_US
dc.identifier.issn0022-2275
dc.identifier.urihttp://hdl.handle.net/11073/25063
dc.description.abstractThe adipocyte-derived secretory protein adiponectin functions as an insulin-sensitizing agent. In plasma, adiponectin exists as low, medium, and high molecular weight oligomers. Treatment with trans-10, cis-12 conjugated linoleic acid (t-10, c-12 CLA) reduces levels of adiponectin as well as triglyceride (TG) in mice and adipocyte cell culture models. The aim of this study was to determine whether the effects of t-10, c-12 CLA on adiponectin and TG are mediated through modulation of the transcription factor peroxisome proliferator-activated receptor γ (PPARγ). 3T3-L1 cells were treated either during or after differentiation into adipocytes with 100 μM t-10, c-12 CLA with or without 10 μM troglitazone, a PPARγ agonist, or 1 μM GW9662, a PPARγ antagonist, and adiponectin and TG levels were analyzed. Treatment with t-10, c-12 CLA reduced TG as well as cellular and secreted adiponectin levels and impaired the assembly of adiponectin oligomers. These changes were accompanied by decreases in PPARγ mass. Troglitazone was able to reverse the t-10, c-12 CLA-mediated decrease in TG levels and restore the assembly of adiponectin oligomers but was unable to restore adiponectin synthesis. Conversely, treatment with GW9662 decreased TG mass and impaired adiponectin oligomer assembly but did not decrease total adiponectin mass. In a reporter assay, t-10, c-12 CLA appeared to be a partial PPARγ agonist and prevented the stimulation of reporter activity by troglitazone. Therefore, the t-10, c-12 CLA isomer appears to alter adipocyte adiponectin metabolism through PPARγ-dependent and PPARγ-independent mechanisms.en_US
dc.description.sponsorshipAdvanced Foods and Materials Networken_US
dc.description.sponsorshipDairy Farmers of Canadaen_US
dc.description.sponsorshipNatural Sciences and Engineering Research Councilen_US
dc.description.sponsorshipHeart and Stroke Foundation of Nova Scotiaen_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.urihttps://doi.org/10.1194/jlr.M700275-JLR200en_US
dc.subjectMouseen_US
dc.subject3T3-L1en_US
dc.subjectAdipocyteen_US
dc.subjectPeroxisome proliferator-activated receptor γen_US
dc.titleThe trans-10, cis-12 isomer of conjugated linoleic acid decreases adiponectin assembly by PPARγ-dependent and PPARγ-independent mechanismsen_US
dc.typeArticleen_US
dc.typePeer-Revieweden_US
dc.typePublished versionen_US
dc.identifier.doi10.1194/jlr.M700275-JLR200


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record