Systems and methods for producing liposomes , including control liposomes and immunoliposomes targeting breast cancer are provided . Systems and methods for treating breast cancer , using targeted immunoliposomes produced according to various methods are also disclosed herein . For example, trastuzumab - conjugated immunoliposomes may be used to deliver chemotherapeutic agents to breast cancer tissues for the treatment of breast cancer . Systems and methods for actuating liposomes using ultrasound are also disclosed , such as systems and methods for actuating trastuzumab - conjugated liposomes accumulated in breast cancer tissues for the treatment of breast cancer.